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| Baculovirus Titration with the Accuri C6 Flow Cytometer 03 Sep 2010: The baculovirus expression vector system (BEVS) is increasingly popular among researchers as a tool for generating large amounts of recombinant protein in insect cells, which post-translationally process proteins in a manner similar to mammalian cells. This technical note demonstrates flow cytometry as a fast, accurate, and often less expensive, more efficient method to determine baculovirus titers. Accuri Cytometers, Inc. |
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| Analyzing Small Particles on the Accuri C6 Flow Cytometer 03 Sep 2010: This technical note demonstrates the method of analyzing small particles on Accuri C6 Flow Cytometer, including setting the threshold, the noise floor and identifying particles of interest. The identification of fluorescent particles and non-fluorescent particles is also discussed. Accuri Cytometers, Inc. |
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| A Guide to Absolute Counting on the Accuri C6 Flow Cytometer 03 Sep 2010: This technical note discusses features of the Accuri C6 Flow Cytometer® for simplifying cell analysis by performing absolute cell counts, eliminating the need for counting beads, or dual-platform hemacytometer counts. The microprocessor-controlled peristaltic pump system allows the sample volume pulled per run to be determined. Accuri Cytometers, Inc. |
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| Multi-Parametric Analysis of Aquatic Organisms Using Flow Cytometry 03 Sep 2010: This application note discusses unique features of the Accuri® C6 Flow Cytometer® System that make it an ideal instrument for aquatic organism analysis. These features include: size, robustness, digital range and versatility. Accuri Cytometers, Inc. |
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| Optimizing Methods in Microtissue Cultivation 02 Sep 2010: This application note shows that InSphero’s GravityPlus technology (patent pending) enables the generation of various microtissue models using either cancer-derived cells to reconstitute tumor tissues or primary cells to mimic structure and function of diverse organs. INTEGRA Biosciences AG |
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| Rapid His-Tag Purification of Recombinant Proteins Using Dionex ProPac IMAC Columns 01 Sep 2010: This application note demonstrates that the Dionex ProPac IMAC column can be used to successfully and rapidly purify His-Tagged recombinant proteins from cell-free extracts harvested from up to 1 L of E. coli fermentation cultures. Purification time may be reduced further through the application of a more concentrated sample with a smaller sample volume. Dionex Corporation |
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| High-Throughput Melt Curve Analysis for Verification and Genotyping of SNPs in 4 Alaska Populations of Chum Salmon 25 Aug 2010: This application poster describes how the BioMark™ System and 96.96 Dynamic Array™ Integrated Fluidic Circuit (IFC) has been used by the University of Washington for verification and genotyping of SNP's in Alaskan populations of Chum Salmon. Fluidigm Corporation |
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| In Vivo Monitoring of Fluorescently Labeled Cancer Cells 18 Aug 2010: This application poster demonstrates that both bioluminescence and fluorescence can be used to measure primary tumor growth and metastasis in vivo. Tumor progressions were monitored non-invasively and in real time using an enclosed imaging system containing a cooled CCD camera. Caliper Life Sciences |
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| Measuring Molecular Mobility with Macro Fluorescence Imaging 18 Aug 2010: This application note examines GFP in live plants and observes systematic differences in areas non-invasively. This demonstrates that molecular mobility can be measured by macro imaging with steady state fluorescence anisotropy with the additional benefits of a large field-of-view which compliments both high throughput, in-vitro assay and in-vivo, non-invasive studies. UVP, LLC. |
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